L- and T-type Ca2+ channels in canine cardiac Purkinje cells. Single-channel demonstration of L-type Ca2+ window current.

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L- and T-type Ca2+ channels in canine cardiac Purkinje cells. Single-channel demonstration of L-type Ca2+ window current.

Canine cardiac Purkinje cells contain both L- and T-type calcium currents, yet the single Ca2+ channels have not been characterized from these cells. Additionally, previous studies have shown an overlap between the steady-state inactivation and activations curves for L-type Ca2+ currents, suggesting the presence of L-type Ca2+ "window" current. We used the on-cell, patch-clamp technique to stud...

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Single-Channel Demonstration of L-Type Ca2+ Window Current

Canine cardiac Purkinje cells contain both Land T-type calcium currents, yet the single Ca2' channels have not been characterized from these cells. Additionally, previous studies have shown an overlap between the steady-state inactivation and activation curves for L-type Ca2' currents, suggesting the presence of L-type Ca2' "window" current. We used the on-cell, patch-clamp technique to study C...

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Potentiated L-type Ca2+ Channels Rectify

Strong depolarization and dihydropyridine agonists potentiate inward currents through native L-type Ca2+ channels, but the effect on outward currents is less clear due to the small size of these currents. Here, we examined potentiation of wild-type alpha1C and two constructs bearing mutations in conserved glutamates in the pore regions of repeats II and IV (E2A/E4A-alpha1C) or repeat III (E3K-a...

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T-type Ca2+ channels in thalamic sensory gating and affective Disorders

Low threshold Ca2+ currents mediated by T-type channels underlie burst spike activities of relay neurons in the thalamus. We have previously reported that knock-out mice for T-type channels show an enhanced nociceptive response to visceral pain, accompanied by an increase in tonic spikes in the absence of burst spikes in thalamic relay neurons. These results raised a possibility that T-type cha...

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Tetrodotoxin Blockade on Canine Cardiac L-Type Ca2+ Channels Depends on pH and Redox Potential

Tetrodotoxin (TTX) is believed to be one of the most selective inhibitors of voltage-gated fast Na⁺ channels in excitable tissues. Recently, however, TTX has been shown to block L-type Ca²⁺ current (I(Ca)) in canine cardiac cells. In the present study, the TTX-sensitivity of I(Ca) was studied in isolated canine ventricular myocytes as a function of (1) channel phosphorylation, (2) extracellular...

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ژورنال

عنوان ژورنال: Circulation Research

سال: 1992

ISSN: 0009-7330,1524-4571

DOI: 10.1161/01.res.70.3.456